If you have Win95-ME. Use a boot disk. If you don't have one get one here.
http://www.bootdisk.com
Then boot with the disk to a DOS prompt. Use the "CD" command to navigate to the directory it's in and delete it. Then reboot.

Also get a good antivirua and run it from boot. Such as norton's. I can be run from the CD at boot to clean an already inffected system. As well, get you a good trojan removal and scanner progy. Such as The Cleaner ( http://www.moosoft.com ) or T Fak ( http://www.kryptocrew.de/snakebyte/tfak.html). You also should restrict the useage and access to the puter. Go to Web Attack ( http://www.webattack.com ) they have a large security section of freeware that will help you.
Hope this helps.